• Our previous high throughput screening HTS campaign using au

  2020-03-09

  

  Our previous high-throughput screening (HTS) campaign using automated, robust, and sensitive fluorescence based arginase inhibitor assay [10], [11] led to the identification of several EPAC specific inhibitors (ESIs), and was subsequently followed by extensive hit-to-lead optimizations [20], [21], [

• br When Does Activity with Alternative Substrates

  2020-03-09

  

  When Does Activity with Alternative Substrates Depend on Neutral Drift? In a final scenario, the alternative reaction catalyzed by an enzyme might have no significant (positive or negative) effects on the system fitness, and thus it would be invisible to natural selection and essentially subject

• In pregnancy maternal uterine spiral artery SA remodeling

  2020-03-09

  

  In pregnancy, maternal uterine spiral artery (SA) remodeling is essential for ensuring efficient blood flow to the developing fetus. A subset of CD49a+Eomes+ decidual NK Medroxyprogesterone acetate synthesis (dNK) that actively secretes GFs, such as pleiotrophin and osteoglycin, have recently been d

• dpni RLS is a genetically complex disorder

  2020-03-09

  

  RLS is a genetically complex disorder in which genetic and environmental factors contribute to the phenotype (Trenkwalder, Allen, Högl, Paulus, & Winkelmann, 2016). This disease is highly familial and six genetic variants with single nucleotide polymorphisms have been identified by genome-wide assoc

• br Cysteine proteases as molecular targets

  2020-03-09

  

  Cysteine proteases as molecular targets for trypanosomatid diseases Approaches relying on a molecular target, such as SBDD and target-based screening, have supported outstanding scientific developments in current pharmaceutical R&D. By integrating experimental and computational methods, these str

• The annotation is arguably the most important part of analys

  2020-03-09

  

  The annotation is arguably the most important part of analysis, as it enables one to evaluate and interpret the content of the transcriptome assembly. In this context, the non-redundant contigs were initially run in BLAST against the Nr database showing that 55% of them displayed high similarity to

• Additionally of outmost important is

  2020-03-09

  

  Additionally, of outmost important is the location of the different CpG islands in relation to the transcription starting site (TSS) of the promoter as well as their interaction with transcription activators or repressors. It has been shown that methylation at the downstream region of the TSS in the

• The final test that was performed was to

  2020-03-09

  

  The final test that was performed was to determine whether the trigger points of the current limiters are stable across a wide temperature range. This test was performed within a thermal-vacuum chamber on each current limiter individually. All the current limiter trip currents were set to approximat

• br Discussion Cytomegalovirus CMV is a herpes

  2020-03-09

  

  Discussion Cytomegalovirus (CMV) is a herpes virus that causes primary infection in normal hosts and re-activation infection in compromised hosts with impaired CMI, e.g., organ transplants. Following primary infection, which may be severe, CMV becomes latent in lymphocytes. Reactivation may occur

• br Acknowledgements This work was supported by a grant from

  2020-03-09

  

  Acknowledgements This work was supported by a grant from the Deutsche Forschungsgemeinschaft to Uwe Knippschild and Martin Wabitsch (SFB 1149/B4). P. Fischer-Posovszky is funded by a Margarete von Wrangell scholarship financed by the Ministerium für Wissenschaft, Forschung und Kunst Baden-Württem

• Rice approximately covers of land area of

  2020-03-09

  

  Rice approximately covers 1% of land area of the earth and it is one of the main sources of food for billions of people [4]. One of the main agriculture industry residues is rice husk, which contains high amount of amorphous silica up to 85–95%. Rice husk ash (RHA) is a solid residue of rice husk co

• Four m thick sections of formalin fixed

  2020-03-09

  

  Four-μm thick sections of formalin-fixed paraffin-embedded tissue samples from uterine cervical cancers were cut with a microtome and dried overnight at 37 °C on a silanized-slide (Dako, Carpentaria, CA, USA). The protocol of the universal Dako Labeled Streptavidin–Biotin kit (Dako) was followed for

• br Experimental section br Author contributions br Acknowled

  2020-03-07

  

  Experimental section Author contributions Acknowledgements Introduction Many EPI-001 in cells are sequential enzymatic reactions where the product of one enzyme serves as the substrate of a second enzyme (1, 2). Experimental evidence suggests that some of these consecutive enzyme-cataly

• Immunofluorescence Cells were fixed with

  2020-03-07

  

  Immunofluorescence. Cells were fixed with 4% paraformaldehyde (PFA) in PBS and extracted by 0.5% Triton X-100-PBS, or fixed in cold methanol. The cells were immunostained with mouse monoclonal Evodiamine against α-tubulin (Sigma), γ-tubulin (Sigma), Ran (Upstate), rabbit polyclonal antibodies again

• All tested antibodies exhibited positive

  2020-03-07

  

  All tested 2-MPPA exhibited positive immunoreactivity in NSCLC tumors, with N’-epitope being the less expressed (both as overall positivity and number of positive cases, or intensity combined with number of positive cells, as provided by H-Score. This result is in accordance with previous studies

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